Construct: shRNA TRCN0000240159

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001130620.1-753s21c1
DNA Barcode:
GATCACATCATGGAGATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RNPS1P1 (643446)
Gene Description:
RNA binding protein with serine rich domain 1 pseudogene 1
Transcript:
RefSeq XM_001130620.1 (NON-CURRENT)
Match location:
Position 753 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240159)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCACATCATGGAGATATTT
Hairpin Sequence:
5'-CCGG-GATCACATCATGGAGATATTT-CTCGAG-AAATATCTCCATGATGTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCACATCATGGAGATATTTCTCGAGAAATATCTCCATGATGTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCACATCATGGAGATATTTCTCGAGAAATATCTCCATGATGTGATC-3'

Other clones with same target sequence:

(none)