Construct: shRNA TRCN0000240195

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001724443.1-3489s21c1
DNA Barcode:
TGAAGAGTAAGGAGTAGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC652522 (652522)
Gene Description:
Werner syndrome ATP-dependent helicase-like
Transcript:
RefSeq XM_001724443.1 (NON-CURRENT)
Match location:
Position 3489 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240195)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGAGTAAGGAGTAGTATT
Hairpin Sequence:
5'-CCGG-TGAAGAGTAAGGAGTAGTATT-CTCGAG-AATACTACTCCTTACTCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGAGTAAGGAGTAGTATTCTCGAGAATACTACTCCTTACTCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGAGTAAGGAGTAGTATTCTCGAGAATACTACTCCTTACTCTTCA-3'

Other clones with same target sequence:

(none)