Construct: shRNA TRCN0000240205

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_977021.1-214s21c1
DNA Barcode:
AGTAATCTCCTACAACCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
EG665449 (665449)
Gene Description:
predicted gene, EG665449
Transcript:
RefSeq XM_977021.1 (NON-CURRENT)
Match location:
Position 214 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240205)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTAATCTCCTACAACCTAAA
Hairpin Sequence:
5'-CCGG-AGTAATCTCCTACAACCTAAA-CTCGAG-TTTAGGTTGTAGGAGATTACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTAATCTCCTACAACCTAAACTCGAGTTTAGGTTGTAGGAGATTACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTAATCTCCTACAACCTAAACTCGAGTTTAGGTTGTAGGAGATTACT-3'

Other clones with same target sequence:

(none)