Construct: shRNA TRCN0000240233

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001473635.1-874s21c1
DNA Barcode:
ACGCAGGTGAAGATCTGGTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nkx1-1 (672284)
Gene Description:
NK1 transcription factor related, locus 1 (Drosophila)
Transcript:
RefSeq XM_001473635.1 (NON-CURRENT)
Match location:
Position 874 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240233)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGCAGGTGAAGATCTGGTTC
Hairpin Sequence:
5'-CCGG-ACGCAGGTGAAGATCTGGTTC-CTCGAG-GAACCAGATCTTCACCTGCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGCAGGTGAAGATCTGGTTCCTCGAGGAACCAGATCTTCACCTGCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGCAGGTGAAGATCTGGTTCCTCGAGGAACCAGATCTTCACCTGCGT-3'

Other clones with same target sequence:

(none)