Construct: shRNA TRCN0000240254
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_001003209.1-689s21c1
- DNA Barcode:
- TTCAAGAGGAAGCCAATATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- LOC676870 (676870)
- Gene Description:
- region containing RIKEN cDNA 2310056B04 gene; pre B-cell leukemia transcription factor 1
- Transcript:
- RefSeq XM_001003209.1 (NON-CURRENT)
- Match location:
- Position 689 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000240254)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TTCAAGAGGAAGCCAATATTT
- Hairpin Sequence:
-
5'-CCGG-TTCAAGAGGAAGCCAATATTT-CTCGAG-AAATATTGGCTTCCTCTTGAA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTTCAAGAGGAAGCCAATATTTCTCGAGAAATATTGGCTTCCTCTTGAATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATTCAAGAGGAAGCCAATATTTCTCGAGAAATATTGGCTTCCTCTTGAA-3'
Other clones with same target sequence:
-
TRCN0000274085
