Construct: shRNA TRCN0000240255

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001003209.1-495s21c1
DNA Barcode:
GAGGCGGAAGAGACGGAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC676870 (676870)
Gene Description:
region containing RIKEN cDNA 2310056B04 gene; pre B-cell leukemia transcription factor 1
Transcript:
RefSeq XM_001003209.1 (NON-CURRENT)
Match location:
Position 495 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240255)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGGCGGAAGAGACGGAATTT
Hairpin Sequence:
5'-CCGG-GAGGCGGAAGAGACGGAATTT-CTCGAG-AAATTCCGTCTCTTCCGCCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGGCGGAAGAGACGGAATTTCTCGAGAAATTCCGTCTCTTCCGCCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGGCGGAAGAGACGGAATTTCTCGAGAAATTCCGTCTCTTCCGCCTC-3'

Other clones with same target sequence:

(none)