Construct: shRNA TRCN0000240278

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001002500.2-3956s21c1
DNA Barcode:
GCATGTTCTTAATCCTATAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp600 (667666)
Gene Description:
zinc finger protein 600
Transcript:
RefSeq XM_001002500.2 (NON-CURRENT)
Match location:
Position 3956 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240278)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGTTCTTAATCCTATAAT
Hairpin Sequence:
5'-CCGG-GCATGTTCTTAATCCTATAAT-CTCGAG-ATTATAGGATTAAGAACATGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGTTCTTAATCCTATAATCTCGAGATTATAGGATTAAGAACATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGTTCTTAATCCTATAATCTCGAGATTATAGGATTAAGAACATGC-3'

Other clones with same target sequence:

(none)