Construct: shRNA TRCN0000240304

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_902322.3-1175s21c1
DNA Barcode:
CAAAGCAGACATCTCCGAATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14326 (665211)
Gene Description:
predicted gene 14326
Transcript:
RefSeq XM_902322.3 (NON-CURRENT)
Match location:
Position 1175 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240304)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAAGCAGACATCTCCGAATA
Hairpin Sequence:
5'-CCGG-CAAAGCAGACATCTCCGAATA-CTCGAG-TATTCGGAGATGTCTGCTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAAGCAGACATCTCCGAATACTCGAGTATTCGGAGATGTCTGCTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAAGCAGACATCTCCGAATACTCGAGTATTCGGAGATGTCTGCTTTG-3'

Other clones with same target sequence:

(none)