Construct: shRNA TRCN0000240321

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001125915.2-277s21c1
DNA Barcode:
GGCCTATGCAAGAACAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC727762 (727762)
Gene Description:
similar to NADH:ubiquinone oxidoreductase B15 subunit
Transcript:
RefSeq XM_001125915.2 (NON-CURRENT)
Match location:
Position 277 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240321)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGCCTATGCAAGAACAATAAA
Hairpin Sequence:
5'-CCGG-GGCCTATGCAAGAACAATAAA-CTCGAG-TTTATTGTTCTTGCATAGGCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGCCTATGCAAGAACAATAAACTCGAGTTTATTGTTCTTGCATAGGCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGCCTATGCAAGAACAATAAACTCGAGTTTATTGTTCTTGCATAGGCC-3'

Other clones with same target sequence:

(none)