Construct: shRNA TRCN0000240330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001093779.2-126s21c1
DNA Barcode:
TTTGCCATCACTGCCATTAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LOC730754 (730754)
Gene Description:
similar to ribosomal protein S18
Transcript:
RefSeq NM_001093779.2 (NON-CURRENT)
Match location:
Position 126 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTTGCCATCACTGCCATTAAG
Hairpin Sequence:
5'-CCGG-TTTGCCATCACTGCCATTAAG-CTCGAG-CTTAATGGCAGTGATGGCAAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTTGCCATCACTGCCATTAAGCTCGAGCTTAATGGCAGTGATGGCAAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTTGCCATCACTGCCATTAAGCTCGAGCTTAATGGCAGTGATGGCAAA-3'

Other clones with same target sequence:

(none)