Construct: shRNA TRCN0000240370

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001479775.1-583s21c1
DNA Barcode:
AGAAGGCGCGACTTATCTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC100047944 (100047944)
Gene Description:
similar to Regulator of G-protein signaling 19
Transcript:
RefSeq XM_001479775.1 (NON-CURRENT)
Match location:
Position 583 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240370)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGGCGCGACTTATCTATG
Hairpin Sequence:
5'-CCGG-AGAAGGCGCGACTTATCTATG-CTCGAG-CATAGATAAGTCGCGCCTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGGCGCGACTTATCTATGCTCGAGCATAGATAAGTCGCGCCTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGGCGCGACTTATCTATGCTCGAGCATAGATAAGTCGCGCCTTCT-3'

Other clones with same target sequence:

(none)