Construct: shRNA TRCN0000240403

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001479470.1-2091s21c1
DNA Barcode:
AGTGAAGGGATAGATTCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC100047704 (100047704)
Gene Description:
similar to chemokine receptor CX3CR1
Transcript:
RefSeq XM_001479470.1 (NON-CURRENT)
Match location:
Position 2091 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240403)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGAAGGGATAGATTCATAT
Hairpin Sequence:
5'-CCGG-AGTGAAGGGATAGATTCATAT-CTCGAG-ATATGAATCTATCCCTTCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGAAGGGATAGATTCATATCTCGAGATATGAATCTATCCCTTCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGAAGGGATAGATTCATATCTCGAGATATGAATCTATCCCTTCACT-3'

Other clones with same target sequence:

(none)