Construct: shRNA TRCN0000240423

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001473573.1-2173s21c1
DNA Barcode:
CTCATGTTGTTCGGTTGTTTC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC100045048 (100045048)
Gene Description:
similar to ortholog of human G protein-coupled receptor 51 GPR51
Transcript:
RefSeq XM_001473573.1 (NON-CURRENT)
Match location:
Position 2173 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240423)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCATGTTGTTCGGTTGTTTC
Hairpin Sequence:
5'-CCGG-CTCATGTTGTTCGGTTGTTTC-CTCGAG-GAAACAACCGAACAACATGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCATGTTGTTCGGTTGTTTCCTCGAGGAAACAACCGAACAACATGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCATGTTGTTCGGTTGTTTCCTCGAGGAAACAACCGAACAACATGAG-3'

Other clones with same target sequence:

(none)