Construct: shRNA TRCN0000240442

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001474490.1-84s21c1
DNA Barcode:
CTTCTTAGTCTACATCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm2707 (100040314)
Gene Description:
predicted gene 2707; MAS-related GPR, member A4 pseudogene
Transcript:
RefSeq XM_001474490.1 (NON-CURRENT)
Match location:
Position 84 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240442)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCTTAGTCTACATCCTAAA
Hairpin Sequence:
5'-CCGG-CTTCTTAGTCTACATCCTAAA-CTCGAG-TTTAGGATGTAGACTAAGAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCTTAGTCTACATCCTAAACTCGAGTTTAGGATGTAGACTAAGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCTTAGTCTACATCCTAAACTCGAGTTTAGGATGTAGACTAAGAAG-3'

Other clones with same target sequence:

(none)