Construct: shRNA TRCN0000240456

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002942.3-3024s21c1
DNA Barcode:
CAGCCATTCGGTCCGTAATAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ROBO2 (6092)
Gene Description:
roundabout guidance receptor 2
Transcript:
RefSeq NM_002942.3 (NON-CURRENT)
Match location:
Position 3024 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240456)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCATTCGGTCCGTAATAA
Hairpin Sequence:
5'-CCGG-CAGCCATTCGGTCCGTAATAA-CTCGAG-TTATTACGGACCGAATGGCTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCATTCGGTCCGTAATAACTCGAGTTATTACGGACCGAATGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCATTCGGTCCGTAATAACTCGAGTTATTACGGACCGAATGGCTG-3'

Other clones with same target sequence:

(none)