Construct: shRNA TRCN0000240462

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014231.3-247s21c1
DNA Barcode:
TACAGCAAACCCAGGCACAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VAMP1 (6843)
Gene Description:
vesicle associated membrane protein 1
Transcript:
RefSeq NM_014231.3 (NON-CURRENT)
Match location:
Position 247 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240462)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACAGCAAACCCAGGCACAAG
Hairpin Sequence:
5'-CCGG-TACAGCAAACCCAGGCACAAG-CTCGAG-CTTGTGCCTGGGTTTGCTGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACAGCAAACCCAGGCACAAGCTCGAGCTTGTGCCTGGGTTTGCTGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACAGCAAACCCAGGCACAAGCTCGAGCTTGTGCCTGGGTTTGCTGTA-3'

Other clones with same target sequence:

(none)