Construct: shRNA TRCN0000240477

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005675.4-320s21c1
DNA Barcode:
CGACGGCACCGTGTTCGAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DGCR6 (8214)
Gene Description:
DiGeorge syndrome critical region gene 6
Transcript:
RefSeq NM_005675.4 (NON-CURRENT)
Match location:
Position 320 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240477)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGACGGCACCGTGTTCGAAAT
Hairpin Sequence:
5'-CCGG-CGACGGCACCGTGTTCGAAAT-CTCGAG-ATTTCGAACACGGTGCCGTCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGACGGCACCGTGTTCGAAATCTCGAGATTTCGAACACGGTGCCGTCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGACGGCACCGTGTTCGAAATCTCGAGATTTCGAACACGGTGCCGTCG-3'

Other clones with same target sequence:

(none)