Construct: shRNA TRCN0000240509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012301.3-577s21c1
DNA Barcode:
TTCGTCACTACCTCAACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MAGI2 (9863)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 2
Transcript:
RefSeq NM_012301.3 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTCGTCACTACCTCAACTTAC
Hairpin Sequence:
5'-CCGG-TTCGTCACTACCTCAACTTAC-CTCGAG-GTAAGTTGAGGTAGTGACGAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTCGTCACTACCTCAACTTACCTCGAGGTAAGTTGAGGTAGTGACGAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTCGTCACTACCTCAACTTACCTCGAGGTAAGTTGAGGTAGTGACGAA-3'

Other clones with same target sequence:

(none)