Construct: shRNA TRCN0000240581

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_007927.2-395s21c1
DNA Barcode:
CTACTATGAGGAGAGTTATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Emd (13726)
Gene Description:
emerin
Transcript:
RefSeq NM_007927.2 (NON-CURRENT)
Match location:
Position 395 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240581)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTACTATGAGGAGAGTTATTT
Hairpin Sequence:
5'-CCGG-CTACTATGAGGAGAGTTATTT-CTCGAG-AAATAACTCTCCTCATAGTAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTACTATGAGGAGAGTTATTTCTCGAGAAATAACTCTCCTCATAGTAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTACTATGAGGAGAGTTATTTCTCGAGAAATAACTCTCCTCATAGTAG-3'

Other clones with same target sequence:

(none)