Construct: shRNA TRCN0000240605

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001999.1-1703s21c1
DNA Barcode:
ACAAGGACCTGCAAACTTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gp1bb (14724)
Gene Description:
glycoprotein Ib, beta polypeptide
Transcript:
RefSeq NM_001001999.1 (CURRENT)
Match location:
Position 1703 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240605)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGGACCTGCAAACTTTAC
Hairpin Sequence:
5'-CCGG-ACAAGGACCTGCAAACTTTAC-CTCGAG-GTAAAGTTTGCAGGTCCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGGACCTGCAAACTTTACCTCGAGGTAAAGTTTGCAGGTCCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGGACCTGCAAACTTTACCTCGAGGTAAAGTTTGCAGGTCCTTGT-3'

Other clones with same target sequence:

(none)