Construct: shRNA TRCN0000240636

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011137.2-1092s21c1
DNA Barcode:
TAAGAACATGTGCAAGTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pou2f1 (18986)
Gene Description:
POU domain, class 2, transcription factor 1
Transcript:
RefSeq NM_011137.2 (NON-CURRENT)
Match location:
Position 1092 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240636)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TAAGAACATGTGCAAGTTAAA
Hairpin Sequence:
5'-CCGG-TAAGAACATGTGCAAGTTAAA-CTCGAG-TTTAACTTGCACATGTTCTTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTAAGAACATGTGCAAGTTAAACTCGAGTTTAACTTGCACATGTTCTTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATAAGAACATGTGCAAGTTAAACTCGAGTTTAACTTGCACATGTTCTTA-3'

Other clones with same target sequence:

(none)