Construct: shRNA TRCN0000240650
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_987021.1-128s21c1
- DNA Barcode:
- AGGACGAGGACGAGGAAGAAG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Nkx1-1 (20232)
- Gene Description:
- NK1 transcription factor related, locus 1 (Drosophila)
- Transcript:
- RefSeq XM_987021.1 (NON-CURRENT)
- Match location:
- Position 128 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000240650)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGGACGAGGACGAGGAAGAAG
- Hairpin Sequence:
-
5'-CCGG-AGGACGAGGACGAGGAAGAAG-CTCGAG-CTTCTTCCTCGTCCTCGTCCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGGACGAGGACGAGGAAGAAGCTCGAGCTTCTTCCTCGTCCTCGTCCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGGACGAGGACGAGGAAGAAGCTCGAGCTTCTTCCTCGTCCTCGTCCT-3'
Other clones with same target sequence:
(none)
