Construct: shRNA TRCN0000240700

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014912.3-1295s21c1
DNA Barcode:
ACCAATGAGTTTCGCTGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPEB3 (22849)
Gene Description:
cytoplasmic polyadenylation element binding protein 3
Transcript:
RefSeq NM_014912.3 (NON-CURRENT)
Match location:
Position 1295 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240700)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCAATGAGTTTCGCTGATAT
Hairpin Sequence:
5'-CCGG-ACCAATGAGTTTCGCTGATAT-CTCGAG-ATATCAGCGAAACTCATTGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCAATGAGTTTCGCTGATATCTCGAGATATCAGCGAAACTCATTGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCAATGAGTTTCGCTGATATCTCGAGATATCAGCGAAACTCATTGGT-3'

Other clones with same target sequence:

(none)