Construct: shRNA TRCN0000240722

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011894.2-1471s21c1
DNA Barcode:
GCCTATGTGCAGATCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sh3bp5 (24056)
Gene Description:
SH3-domain binding protein 5 (BTK-associated)
Transcript:
RefSeq NM_011894.2 (NON-CURRENT)
Match location:
Position 1471 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240722)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTATGTGCAGATCATATTT
Hairpin Sequence:
5'-CCGG-GCCTATGTGCAGATCATATTT-CTCGAG-AAATATGATCTGCACATAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTATGTGCAGATCATATTTCTCGAGAAATATGATCTGCACATAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTATGTGCAGATCATATTTCTCGAGAAATATGATCTGCACATAGGC-3'

Other clones with same target sequence:

(none)