Construct: shRNA TRCN0000240738

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014033.3-409s21c1
DNA Barcode:
GTGAGGTTCACTGTGATATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
METTL7A (25840)
Gene Description:
methyltransferase like 7A
Transcript:
RefSeq NM_014033.3 (NON-CURRENT)
Match location:
Position 409 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240738)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGGTTCACTGTGATATAC
Hairpin Sequence:
5'-CCGG-GTGAGGTTCACTGTGATATAC-CTCGAG-GTATATCACAGTGAACCTCAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGGTTCACTGTGATATACCTCGAGGTATATCACAGTGAACCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGGTTCACTGTGATATACCTCGAGGTATATCACAGTGAACCTCAC-3'

Other clones with same target sequence:

(none)