Construct: shRNA TRCN0000240761

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031413.2-5384s21c1
DNA Barcode:
GCTAGAGTGTTTCACGTATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CECR2 (27443)
Gene Description:
CECR2 histone acetyl-lysine reader
Transcript:
RefSeq NM_031413.2 (NON-CURRENT)
Match location:
Position 5384 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240761)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGAGTGTTTCACGTATTA
Hairpin Sequence:
5'-CCGG-GCTAGAGTGTTTCACGTATTA-CTCGAG-TAATACGTGAAACACTCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGAGTGTTTCACGTATTACTCGAGTAATACGTGAAACACTCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGAGTGTTTCACGTATTACTCGAGTAATACGTGAAACACTCTAGC-3'

Other clones with same target sequence:

(none)