Construct: shRNA TRCN0000240801

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015823.2-4154s21c1
DNA Barcode:
TGTCACACGCATAGATCTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Magi2 (50791)
Gene Description:
membrane associated guanylate kinase, WW and PDZ domain containing 2
Transcript:
RefSeq NM_015823.2 (NON-CURRENT)
Match location:
Position 4154 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240801)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGTCACACGCATAGATCTATA
Hairpin Sequence:
5'-CCGG-TGTCACACGCATAGATCTATA-CTCGAG-TATAGATCTATGCGTGTGACA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGTCACACGCATAGATCTATACTCGAGTATAGATCTATGCGTGTGACATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGTCACACGCATAGATCTATACTCGAGTATAGATCTATGCGTGTGACA-3'

Other clones with same target sequence:

(none)