Construct: shRNA TRCN0000240835

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081279.1-5579s21c1
DNA Barcode:
GTTCAATGTCCGCTATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mfhas1 (52065)
Gene Description:
malignant fibrous histiocytoma amplified sequence 1
Transcript:
RefSeq NM_001081279.1 (CURRENT)
Match location:
Position 5579 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240835)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTTCAATGTCCGCTATGATTT
Hairpin Sequence:
5'-CCGG-GTTCAATGTCCGCTATGATTT-CTCGAG-AAATCATAGCGGACATTGAAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTTCAATGTCCGCTATGATTTCTCGAGAAATCATAGCGGACATTGAACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTTCAATGTCCGCTATGATTTCTCGAGAAATCATAGCGGACATTGAAC-3'

Other clones with same target sequence:

(none)