Construct: shRNA TRCN0000240838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_177469.3-2033s21c1
DNA Barcode:
ACTGGGACTCTTTGTACTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Adgra1 (52389)
Gene Description:
adhesion G protein-coupled receptor A1
Transcript:
RefSeq NM_177469.3 (CURRENT)
Match location:
Position 2033 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTGGGACTCTTTGTACTTAT
Hairpin Sequence:
5'-CCGG-ACTGGGACTCTTTGTACTTAT-CTCGAG-ATAAGTACAAAGAGTCCCAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTGGGACTCTTTGTACTTATCTCGAGATAAGTACAAAGAGTCCCAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTGGGACTCTTTGTACTTATCTCGAGATAAGTACAAAGAGTCCCAGT-3'

Other clones with same target sequence:

(none)