Construct: shRNA TRCN0000240855

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019867.2-1612s21c1
DNA Barcode:
TGACCGAGAGGCCACCTATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ngef (53972)
Gene Description:
neuronal guanine nucleotide exchange factor
Transcript:
RefSeq NM_019867.2 (CURRENT)
Match location:
Position 1612 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240855)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGACCGAGAGGCCACCTATAT
Hairpin Sequence:
5'-CCGG-TGACCGAGAGGCCACCTATAT-CTCGAG-ATATAGGTGGCCTCTCGGTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGACCGAGAGGCCACCTATATCTCGAGATATAGGTGGCCTCTCGGTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGACCGAGAGGCCACCTATATCTCGAGATATAGGTGGCCTCTCGGTCA-3'

Other clones with same target sequence:

(none)