Construct: shRNA TRCN0000240865

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019051.2-860s21c1
DNA Barcode:
TCATCCTGTTAGGATTCATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRPL50 (54534)
Gene Description:
mitochondrial ribosomal protein L50
Transcript:
RefSeq NM_019051.2 (NON-CURRENT)
Match location:
Position 860 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240865)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATCCTGTTAGGATTCATAT
Hairpin Sequence:
5'-CCGG-TCATCCTGTTAGGATTCATAT-CTCGAG-ATATGAATCCTAACAGGATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATCCTGTTAGGATTCATATCTCGAGATATGAATCCTAACAGGATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATCCTGTTAGGATTCATATCTCGAGATATGAATCCTAACAGGATGA-3'

Other clones with same target sequence:

(none)