Construct: shRNA TRCN0000240878

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001037533.1-1304s21c1
DNA Barcode:
GATGAGGAGAGTGGCATATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GON4L (54856)
Gene Description:
gon-4 like
Transcript:
RefSeq NM_001037533.1 (NON-CURRENT)
Match location:
Position 1304 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240878)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATGAGGAGAGTGGCATATTA
Hairpin Sequence:
5'-CCGG-GATGAGGAGAGTGGCATATTA-CTCGAG-TAATATGCCACTCTCCTCATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATGAGGAGAGTGGCATATTACTCGAGTAATATGCCACTCTCCTCATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATGAGGAGAGTGGCATATTACTCGAGTAATATGCCACTCTCCTCATC-3'

Other clones with same target sequence:

(none)