Construct: shRNA TRCN0000240887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017869.3-381s21c1
DNA Barcode:
GCCCTGGAGGCTACTTGTAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BANP (54971)
Gene Description:
BTG3 associated nuclear protein
Transcript:
RefSeq NM_017869.3 (NON-CURRENT)
Match location:
Position 381 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000240887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTGGAGGCTACTTGTAAA
Hairpin Sequence:
5'-CCGG-GCCCTGGAGGCTACTTGTAAA-CTCGAG-TTTACAAGTAGCCTCCAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTGGAGGCTACTTGTAAACTCGAGTTTACAAGTAGCCTCCAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTGGAGGCTACTTGTAAACTCGAGTTTACAAGTAGCCTCCAGGGC-3'

Other clones with same target sequence:

(none)