Construct: shRNA TRCN0000241040

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001042499.1-414s21c1
DNA Barcode:
CCGAGAACAGTTTGCTGATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rabl3 (67657)
Gene Description:
RAB, member RAS oncogene family-like 3
Transcript:
RefSeq NM_001042499.1 (CURRENT)
Match location:
Position 414 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241040)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGAACAGTTTGCTGATAA
Hairpin Sequence:
5'-CCGG-CCGAGAACAGTTTGCTGATAA-CTCGAG-TTATCAGCAAACTGTTCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGAACAGTTTGCTGATAACTCGAGTTATCAGCAAACTGTTCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGAACAGTTTGCTGATAACTCGAGTTATCAGCAAACTGTTCTCGG-3'

Other clones with same target sequence:

(none)