Construct: shRNA TRCN0000241111

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026738.2-556s21c1
DNA Barcode:
CCTTCTCACCTGTCATCATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Card19 (68480)
Gene Description:
caspase recruitment domain family, member 19
Transcript:
RefSeq NM_026738.2 (CURRENT)
Match location:
Position 556 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241111)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTCTCACCTGTCATCATTG
Hairpin Sequence:
5'-CCGG-CCTTCTCACCTGTCATCATTG-CTCGAG-CAATGATGACAGGTGAGAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTCTCACCTGTCATCATTGCTCGAGCAATGATGACAGGTGAGAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTCTCACCTGTCATCATTGCTCGAGCAATGATGACAGGTGAGAAGG-3'

Other clones with same target sequence:

(none)