Construct: shRNA TRCN0000241222

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027434.2-799s21c1
DNA Barcode:
GTCTGTTACTAGCGGAATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rprd1b (70470)
Gene Description:
regulation of nuclear pre-mRNA domain containing 1B
Transcript:
RefSeq NM_027434.2 (NON-CURRENT)
Match location:
Position 799 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241222)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTGTTACTAGCGGAATATA
Hairpin Sequence:
5'-CCGG-GTCTGTTACTAGCGGAATATA-CTCGAG-TATATTCCGCTAGTAACAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTGTTACTAGCGGAATATACTCGAGTATATTCCGCTAGTAACAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTGTTACTAGCGGAATATACTCGAGTATATTCCGCTAGTAACAGAC-3'

Other clones with same target sequence:

(none)