Construct: shRNA TRCN0000241290

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028032.2-916s21c1
DNA Barcode:
GCAGATGATTTGCGAATTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ppp2r2a (71978)
Gene Description:
protein phosphatase 2, regulatory subunit B, alpha
Transcript:
RefSeq NM_028032.2 (NON-CURRENT)
Match location:
Position 916 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241290)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGATTTGCGAATTAAT
Hairpin Sequence:
5'-CCGG-GCAGATGATTTGCGAATTAAT-CTCGAG-ATTAATTCGCAAATCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGATTTGCGAATTAATCTCGAGATTAATTCGCAAATCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGATTTGCGAATTAATCTCGAGATTAATTCGCAAATCATCTGC-3'

Other clones with same target sequence:

(none)