Construct: shRNA TRCN0000241324

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028307.1-170s21c1
DNA Barcode:
CATTCTGTATCGTCGATATAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tdrkh (72634)
Gene Description:
tudor and KH domain containing protein
Transcript:
RefSeq NM_028307.1 (CURRENT)
Match location:
Position 170 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241324)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATTCTGTATCGTCGATATAG
Hairpin Sequence:
5'-CCGG-CATTCTGTATCGTCGATATAG-CTCGAG-CTATATCGACGATACAGAATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATTCTGTATCGTCGATATAGCTCGAGCTATATCGACGATACAGAATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATTCTGTATCGTCGATATAGCTCGAGCTATATCGACGATACAGAATG-3'

Other clones with same target sequence:

(none)