Construct: shRNA TRCN0000241371

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028709.1-3383s21c1
DNA Barcode:
GTCTCGTAGTTTCGAATATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Btbd11 (74007)
Gene Description:
BTB (POZ) domain containing 11
Transcript:
RefSeq NM_028709.1 (NON-CURRENT)
Match location:
Position 3383 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241371)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCTCGTAGTTTCGAATATAT
Hairpin Sequence:
5'-CCGG-GTCTCGTAGTTTCGAATATAT-CTCGAG-ATATATTCGAAACTACGAGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCTCGTAGTTTCGAATATATCTCGAGATATATTCGAAACTACGAGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCTCGTAGTTTCGAATATATCTCGAGATATATTCGAAACTACGAGAC-3'

Other clones with same target sequence:

(none)