Construct: shRNA TRCN0000241373

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028720.2-384s21c1
DNA Barcode:
ATGACAAGAATCGGCGTAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Glyr1 (74022)
Gene Description:
glyoxylate reductase 1 homolog (Arabidopsis)
Transcript:
RefSeq NM_028720.2 (CURRENT)
Match location:
Position 384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241373)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACAAGAATCGGCGTAATT
Hairpin Sequence:
5'-CCGG-ATGACAAGAATCGGCGTAATT-CTCGAG-AATTACGCCGATTCTTGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACAAGAATCGGCGTAATTCTCGAGAATTACGCCGATTCTTGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACAAGAATCGGCGTAATTCTCGAGAATTACGCCGATTCTTGTCAT-3'

Other clones with same target sequence:

(none)