Construct: shRNA TRCN0000241410

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_028956.4-1822s21c1
DNA Barcode:
GATCTATTTGTACGCATATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pus10 (74467)
Gene Description:
pseudouridylate synthase 10
Transcript:
RefSeq NM_028956.4 (CURRENT)
Match location:
Position 1822 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241410)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCTATTTGTACGCATATTT
Hairpin Sequence:
5'-CCGG-GATCTATTTGTACGCATATTT-CTCGAG-AAATATGCGTACAAATAGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCTATTTGTACGCATATTTCTCGAGAAATATGCGTACAAATAGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCTATTTGTACGCATATTTCTCGAGAAATATGCGTACAAATAGATC-3'

Other clones with same target sequence:

  1. TRCN0000216556