Construct: shRNA TRCN0000241432

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181815.3-2393s21c1
DNA Barcode:
CCGAGCAGATCTCGCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cep128 (75216)
Gene Description:
centrosomal protein 128
Transcript:
RefSeq NM_181815.3 (CURRENT)
Match location:
Position 2393 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241432)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGCAGATCTCGCTAATAA
Hairpin Sequence:
5'-CCGG-CCGAGCAGATCTCGCTAATAA-CTCGAG-TTATTAGCGAGATCTGCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGCAGATCTCGCTAATAACTCGAGTTATTAGCGAGATCTGCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGCAGATCTCGCTAATAACTCGAGTTATTAGCGAGATCTGCTCGG-3'

Other clones with same target sequence:

(none)