Construct: shRNA TRCN0000241470

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081045.1-2060s21c1
DNA Barcode:
CAAACAGATACGTGCTAATAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Kansl1 (76719)
Gene Description:
KAT8 regulatory NSL complex subunit 1
Transcript:
RefSeq NM_001081045.1 (CURRENT)
Match location:
Position 2060 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241470)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAACAGATACGTGCTAATAA
Hairpin Sequence:
5'-CCGG-CAAACAGATACGTGCTAATAA-CTCGAG-TTATTAGCACGTATCTGTTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAACAGATACGTGCTAATAACTCGAGTTATTAGCACGTATCTGTTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAACAGATACGTGCTAATAACTCGAGTTATTAGCACGTATCTGTTTG-3'

Other clones with same target sequence:

  1. TRCN0000369878