Construct: shRNA TRCN0000241488

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001024842.1-705s21c1
DNA Barcode:
GCGCTGCCCTTATTCGAAATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hoxc11 (109663)
Gene Description:
homeobox C11
Transcript:
RefSeq NM_001024842.1 (CURRENT)
Match location:
Position 705 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241488)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGCTGCCCTTATTCGAAATT
Hairpin Sequence:
5'-CCGG-GCGCTGCCCTTATTCGAAATT-CTCGAG-AATTTCGAATAAGGGCAGCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGCTGCCCTTATTCGAAATTCTCGAGAATTTCGAATAAGGGCAGCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGCTGCCCTTATTCGAAATTCTCGAGAATTTCGAATAAGGGCAGCGC-3'

Other clones with same target sequence:

  1. TRCN0000020413
  2. TRCN0000189990