Construct: shRNA TRCN0000241512

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026398.4-785s21c1
DNA Barcode:
ACTTGTGGGAACAGGACATTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pop5 (117109)
Gene Description:
processing of precursor 5, ribonuclease P/MRP family (S. cerevisiae)
Transcript:
RefSeq NM_026398.4 (CURRENT)
Match location:
Position 785 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241512)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTTGTGGGAACAGGACATTA
Hairpin Sequence:
5'-CCGG-ACTTGTGGGAACAGGACATTA-CTCGAG-TAATGTCCTGTTCCCACAAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTTGTGGGAACAGGACATTACTCGAGTAATGTCCTGTTCCCACAAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTTGTGGGAACAGGACATTACTCGAGTAATGTCCTGTTCCCACAAGT-3'

Other clones with same target sequence:

(none)