Construct: shRNA TRCN0000241572

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178868.3-889s21c1
DNA Barcode:
ATATTCCCAGAGAATTGTATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CMTM8 (152189)
Gene Description:
CKLF like MARVEL transmembrane domain containing 8
Transcript:
RefSeq NM_178868.3 (NON-CURRENT)
Match location:
Position 889 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241572)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATTCCCAGAGAATTGTATT
Hairpin Sequence:
5'-CCGG-ATATTCCCAGAGAATTGTATT-CTCGAG-AATACAATTCTCTGGGAATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATTCCCAGAGAATTGTATTCTCGAGAATACAATTCTCTGGGAATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATTCCCAGAGAATTGTATTCTCGAGAATACAATTCTCTGGGAATAT-3'

Other clones with same target sequence:

(none)