Construct: shRNA TRCN0000241578

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_138492.4-749s21c1
DNA Barcode:
AGGGAGCCCAGAAGGGAATTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PRELID2 (153768)
Gene Description:
PRELI domain containing 2
Transcript:
RefSeq NM_138492.4 (NON-CURRENT)
Match location:
Position 749 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241578)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGGAGCCCAGAAGGGAATTA
Hairpin Sequence:
5'-CCGG-AGGGAGCCCAGAAGGGAATTA-CTCGAG-TAATTCCCTTCTGGGCTCCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGGAGCCCAGAAGGGAATTACTCGAGTAATTCCCTTCTGGGCTCCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGGAGCCCAGAAGGGAATTACTCGAGTAATTCCCTTCTGGGCTCCCT-3'

Other clones with same target sequence:

(none)