Construct: shRNA TRCN0000241610

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_027748.2-800s21c1
DNA Barcode:
CACGACTCCTGAGCACTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Taf3 (209361)
Gene Description:
TATA-box binding protein associated factor 3
Transcript:
RefSeq NM_027748.2 (NON-CURRENT)
Match location:
Position 800 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241610)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACGACTCCTGAGCACTAAAG
Hairpin Sequence:
5'-CCGG-CACGACTCCTGAGCACTAAAG-CTCGAG-CTTTAGTGCTCAGGAGTCGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACGACTCCTGAGCACTAAAGCTCGAGCTTTAGTGCTCAGGAGTCGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACGACTCCTGAGCACTAAAGCTCGAGCTTTAGTGCTCAGGAGTCGTG-3'

Other clones with same target sequence:

(none)