Construct: shRNA TRCN0000241612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001081278.2-1137s21c1
DNA Barcode:
CTCACCTACTTTGCCTATTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tbc1d4 (210789)
Gene Description:
TBC1 domain family, member 4
Transcript:
RefSeq NM_001081278.2 (CURRENT)
Match location:
Position 1137 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCACCTACTTTGCCTATTTG
Hairpin Sequence:
5'-CCGG-CTCACCTACTTTGCCTATTTG-CTCGAG-CAAATAGGCAAAGTAGGTGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCACCTACTTTGCCTATTTGCTCGAGCAAATAGGCAAAGTAGGTGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCACCTACTTTGCCTATTTGCTCGAGCAAATAGGCAAAGTAGGTGAG-3'

Other clones with same target sequence:

(none)