Construct: shRNA TRCN0000241794

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198192.2-1565s21c1
DNA Barcode:
TATCCATGTAGTCAATGTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Qrfpr (229214)
Gene Description:
pyroglutamylated RFamide peptide receptor
Transcript:
RefSeq NM_198192.2 (CURRENT)
Match location:
Position 1565 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241794)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TATCCATGTAGTCAATGTAAT
Hairpin Sequence:
5'-CCGG-TATCCATGTAGTCAATGTAAT-CTCGAG-ATTACATTGACTACATGGATA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTATCCATGTAGTCAATGTAATCTCGAGATTACATTGACTACATGGATATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATATCCATGTAGTCAATGTAATCTCGAGATTACATTGACTACATGGATA-3'

Other clones with same target sequence:

(none)